Ray B. Nagle

Professor and Associate Head
Department of Pathology and Cell Biology and Anatomy
M.D., Ph.D., The University of Washington, Seattle

email address: rnagle@u.arizona.edu

Pathogeneis of human cancer

The work in my laboratory primarily focuses on the interaction of cell surface molecules with the extracellular matrix, and migration of cells.  These problems are basic to our understanding of the invasion and spread of cancer.  We have focused on prostate cancer and use a variety of approaches including protein chemistry, immunohistochemical analysis, ultrastructural analysis, and molecular procedures.  Our work includes model systems using prostate cell lines, immunodeficient mice, and human tissues.  Opportunities to learn technical procedures include monoclonal antibody production, transfection (using cDNA), and analysis of messenger RNA at the cellular level using in-situ hybridization.

Yang Y, Hao J, Liu X, Dalkin B, Nagle RB (1997)  Differential expression of cytokeratin mRNA and protein in normal prostate, prostate eipthelial neoplasia, and invasive carcinoma.  Am J Pathol, 150:693-704.

von Bredow D, Nagle RB, Bowden GT, Cress AE (1997) Cleavage of B4 integrin by matrilysin.  Exp Cell Res, 236:341-345.

von Bredow DC, Cress AE, Howard EQ, Bowden GT, Nagle RB (1998) Activation of gelatinase/tissue inhibitors of metalloproteinase complexes by matrilysin.  Biochemical Journal 331:965-972.

Knox JD, Breton L, Lynch T, Bowden GT, Nagle RB (1998) Synthetic matrix metalloproteinase inhibitor, BB-94, inhibits the invasion of neoplastic human prostate cells in a mouse model.  The Prostate 35:248-254.

Gupta A, Andrews KL, McDaniel KM, Nagle RB, Bowden GT (1999) Experimental induction of rhabdomyosarcoma in mice with fractionated doses of B-irradiation. J Cancer Res Clin Oncol 125:257-267.

Sundareshan P, Nagle RB, Bowden GT (1999) EGF induces the expression of matrilysin in the human prostate adenocarcinoma cell line, LNCaP.  The Prostate 40:159-166.